Acetobacter diazotrophicus Agar

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetobacter diazotrophicus Agar
Composition per liter:
Glucose.......................................................................................50.0g
CaCO 3 .........................................................................................30.0g
Agar............................................................................................25.0g
Yeast extract................................................................................10.0g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly to evenly distribute
CaCO 3 . Bring pH to 5.5. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Cool rapidly to 50°–55°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the cultivation and maintenance of Acetobacter diazotrophi-
cus.