Acetobacter Broth

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetobacter Broth
Composition per liter:
Yeast extract..................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................3.3g
KH 2 PO 4 ........................................................................................1.0g
MgSO 4 ........................................................................................0.25g
Vitamin solution.....................................................................200.0mL
Glucose solution ......................................................................15.0mL
Trace elements solution .............................................................1.0mL
Trace Elements Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O..............................................................................1.457g
FeSO 4 ·7H 2 O .............................................................................0.366g
ZnSO 4 ·7H 2 O.............................................................................0.178g
MnSO 4 ·H 2 O..............................................................................0.101g
Na 2 MoO 4 ·2H 2 O.......................................................................23.4mg
CuSO 4 ·5H 2 O..............................................................................7.8mg
Preparation of Trace Elements Solution:  Add components to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Vitamin Solution:
Composition per 100.0mL:
m-Inositol...............................................................................200.0mg
Calcium  DL -pantothenate.........................................................40.0mg
Nicotinic acid...........................................................................40.0mg
Pyrdoxine·HCl.........................................................................40.0mg
Thiamine·HCl..........................................................................40.0mg
p-Aminobenzoic acid...............................................................20.0mg
Riboflavin................................................................................20.0mg
Biotin.........................................................................................0.2mg
Folic acid ...................................................................................0.2mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................40.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solution,
glucose solution, and trace elements solution, to distilled/deionized water
and bring volume to 784.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°–55°C. Aseptically add 200.0mL of sterile
vitamin solution, 15.0mL of sterile glucose solution, and 1.0mL of sterile
trace elments solution. Mix thoroughly. Aseptically distribute into sterile
tubes or flasks.
Use:  For the cultivation of Acetobacter xylinum.