Halophilic Nutrient Agar (LMG Medium 220)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halophilic Nutrient Agar

(LMG Medium 220)

培养基配方

Composition per liter:
NaCl............................................................................................60.0g
Agar ............................................................................................15.0g
Casein peptone, tryptic digest.....................................................10.0g
Yeast extract..................................................................................5.0g
Glucose .........................................................................................5.0g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to tap water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of halophilic bacteria.