Halophilic Methanotrophic Bacterium Medium (DSMZ Medium 1156)

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halophilic Methanotrophic Bacterium Medium

(DSMZ Medium 1156)

培养基配方

Composition per liter:
NaCl............................................................................................65.0g
Na 2 HPO 4 · 2H 2 O.............................................................................0.7g
NH 4 Cl ..........................................................................................0.5g
KH 2 PO 4 ........................................................................................0.3g
MgSO 4 · 7H 2 O................................................................................0.1g
CaCl 2 ·2H 2 O ................................................................................0.01g
FeSO 4 ·7H 2 O.............................................................................. 5.0mg
Methanol....................................................................................5.0mL
Trace elements solution.............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
ZnSO 4 ·7H 2 O.............................................................................. 0.44g
CuSO 4 ·5H 2 O.................................................................................0.2g
MnSO 4 ·2H 2 O..............................................................................0.17g
H 3 BO 3 ...........................................................................................0.1g
CoCl 2 ·6H 2 O................................................................................0.08g
Na 2 MoO 4 ·2H 2 O......................................................................... 0.06g
Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.6 with 10% NaOH. Gently heat while stirring and bring to boiling. Distribute 25.0mL aliquots into 120mL serum bottles closed with butyl rubber serum caps and aluminum crimps. Autoclave for 15 min at 15 psi pressure–121°C. The pH is critical and the final pH must be 6.5–7.5. Some insoluble phosphates may precipitate during medium preparation (the turbidity should not be more than OD 0.005). Instead of methanol a headspace with 20% methane, 2% CO 2 , and 78% air can be used.
Use: For the cultivation of Methylohalobius crimeensis.