Halophile Agar I

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halophile Agar I

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
Casamino acids.............................................................................7.5g
Trisodium citrate...........................................................................3.0g
KCl................................................................................................2.0g
FeSO 4 ·7H 2 O ...............................................................................0.05g
MnSO 4 ·4H 2 O...........................................................................0.25mg
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Add agar. Gently heat and bring to boiling. Do not autoclave. Sterilize by steaming at 100°C for 30 min. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Halobacterium halobium, Halobacterium salinarium, and Halobacterium trapanicum.