Halophile Agar

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halophile Agar

培养基配方

Composition per liter:
NaCl..........................................................................................156.0g
Agar............................................................................................20.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
MgCl 2 ·6H 2 O ...............................................................................13.0g
Yeast extract................................................................................10.0g
KCl................................................................................................4.0g
CaCl 2 ·6H 2 O ..................................................................................1.0g
NaBr..............................................................................................0.5g
NaHCO 3 ........................................................................................0.2g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Haloarcula hispanica, Haloferax gibbonsii, Marinococcus halophilus, Planococcus species, and Sporosarcina halophila.