Halobacteriaceae Medium 4

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacteriaceae Medium 4

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
NH 4 Cl ...........................................................................................5.0g
L- Glutamic acid.............................................................................1.3g
DL- Valine.......................................................................................1.0g
Glycerol ........................................................................................1.0g
L- Lysine.......................................................................................0.85g
L- Leucine ......................................................................................0.8g
DL- Serine.....................................................................................0.61g
DL- Threonine.................................................................................0.5g
DL- Isoleucine...............................................................................0.44g
DL- Alanine ..................................................................................0.43g
L- Arginine.....................................................................................0.4g
DL- Methionine.............................................................................0.37g
DL- Phenylalanine ........................................................................0.26g
L- Tyrosine .....................................................................................0.2g
Adenylic acid................................................................................0.1g

KNO 3 ............................................................................................0.1g

Uridylic acid .................................................................................0.1g
Glycine........................................................................................0.06g
KH 2 PO 4 .......................................................................................0.05g
K 2 HPO 4 .......................................................................................0.05g
L- Cysteine....................................................................................0.05g
L- Proline......................................................................................0.05g
Sodium citrate.............................................................................0.05g
FeCl 2 ..........................................................................................2.3mg
CaCl 2 ·2H 2 O................................................................................0.7mg
ZnSO 4 ·7H 2 O............................................................................0.44mg
MnSO 4 ·H 2 O...............................................................................0.3mg
CuSO 4 ·5H 2 O............................................................................0.05mg
pH 6.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 6.2. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of members of the Halobacteriaceae.