Halobacteriaceae Medium 3

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacteriaceae Medium 3

培养基配方

Composition per liter:
NaCl..........................................................................................240.0g
L- Glutamine ................................................................................15.0g
KCl................................................................................................5.0g
K 2 SO 4 ...........................................................................................5.0g
MgCl 2 ·6H 2 O .................................................................................5.0g
MgSO 4 , anhydrous........................................................................5.0g
NH 4 Cl ...........................................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................0.5g
L- Arginine.....................................................................................0.5g
L- Isoleucine.................................................................................0.25g
L- Leucine ....................................................................................0.25g
L- Lysine.......................................................................................0.25g
L- Proline......................................................................................0.25g
L- Valine.......................................................................................0.25g
Cytidylic acid................................................................................0.2g
CaCl 2 ·2H 2 O ..................................................................................0.1g
L- Methionine.................................................................................0.1g
L- Tyrosine .....................................................................................0.1g
L- Phenylalanine...........................................................................0.05g
FeCl 2 ·6H 2 O................................................................................5.0mg
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat until dissolved. Adjust pH to 6.8. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of some halobacteria and halococci.