GYPT Medium (Glucose Yeast Extract Peptone Thioglycolate Medium)

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYPT Medium
(Glucose Yeast Extract Peptone

Thioglycolate Medium)

培养基配方

Composition per liter:
Agar ..............................................................................................8.0g
Yeast extract..................................................................................6.0g
Glucose .........................................................................................5.0g
Peptone..........................................................................................2.0g
Sodium thioglycolate....................................................................0.5g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Gently heat and bring to boiling. Anaerobically distribute into tubes under 97% N 2 + 3% H 2 . Cap tubes with rubber stoppers. Place tubes in a press. Autoclave for 15 min at 15 psi pressure–121°C with fast exhaust.
Use: For the cultivation of Spirochaeta stenostrepta.