GYP Sodium Acetate Mineral Salts Broth with 5% Sodium Chloride (LMG Medium 244)

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYP Sodium Acetate Mineral
Salts Broth with 5% Sodium Chloride

(LMG Medium 244)

培养基配方

Composition per liter:
NaCl............................................................................................50.0g
Glucose .......................................................................................10.0g
Yeast extract................................................................................10.0g
Peptone........................................................................................10.0g
Sodium acetate............................................................................10.0g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O...........................................................................10.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
pH 6.8± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Tetragenococcus halophilus and Tetragenococcus muriaticus.