GYP Agar

[所属分类:培养基配方] [发布时间:2021-3-22] [发布人: ] [阅读次数:] [返回]

GYP Agar

培养基配方

Composition per liter:
Glucose.......................................................................................40.0g
Agar............................................................................................20.0g
Peptone .......................................................................................20.0g
Sodium acetate............................................................................20.0g
Yeast extract................................................................................20.0g
Solution A................................................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Solution A:
Composition per 100.0mL:
MgSO 4 ·7H 2 O................................................................................4.0g
FeSO 4 ·7H 2 O..................................................................................0.2g
MnSO 4 ·H 2 O..................................................................................0.2g
NaCl..............................................................................................0.2g
Preparation of Solution A:  Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Bacillus laevolacticus, Bacillus racemilacticus, and Sporolactobacillus inulinus.