ABY Agar (Acid Bismuth Yeast Agar)

[所属分类:培养基配方] [发布时间:2019-9-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ABY Agar
(Acid Bismuth Yeast Agar)
Composition per liter:
Agar ............................................................................................20.0g
Glucose .......................................................................................20.0g
Bi 2 (SO 3 ) 2 .......................................................................................8.0g
(NH 4 ) 2 SO 4 .....................................................................................3.0g
KH 2 PO 4 .........................................................................................3.0g
MgSO 4 ·7H 2 O..............................................................................0.25g
CaCl 2 ·2H 2 O.................................................................................0.25g
Biotin ........................................................................................10.0μg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Cool tubes in a slanted position.
Use: For the selective isolation and differentiation of Candida albi-
cans from other Candida species. Candida albicans and Candida trop-
icalis colonies appear as smooth, brownish-black round colonies.
Other Candida species are differentially pigmented or produce diffus-
ible pigments. Usually used in conjunction with BiGGY agar to differ-
entiate further Candida; on BiGGY agar, Candida albicans appears as
brown to black colonies with no pigment diffusion and no sheen,
whereas Candida tropicalis appears as dark brown colonies with black
centers, black pigment diffusion, and a sheen.