Abeyta-Hunt Bark Agar

[所属分类:培养基配方] [发布时间:2019-9-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Abeyta-Hunt Bark Agar
Composition per 1016.0mL:
Beef heart, infusion from..........................................................500.0g
Agar............................................................................................15.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................2.0g
Horse blood, lysed...................................................................50.0mL
Cefoperazone solution...............................................................4.0mL
Rifampicin solution ...................................................................4.0mL
Amphotericin B solution............................................................4.0mL
Ferrous sulfate pyruvate
metabisulfite solution..........................................................4.0mL
pH 7.4 ± 0.2 at 25°C
© 2010 by Taylor and Francis Group, LLC
AC HiVeg Agar  17
Amphotericin B Solution:
Composition per 100.0mL:
Amphotericin B...........................................................................0.05g
Preparation of Amphotericin B Solution: Add amphotericin B
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Cefoperazone Solution:
Composition per 100.0mL:
Sodium cefoperazone..................................................................0.08g
Preparation of Cefoperazone Solution: Add sodium cefopera-
zone to distilled/deionized water and bring volume to 100.0mL. Mix
thoroughly. Filter sterilize.
Rifampicin Solution:
Composition per 100.0mL
Rifampicin ..................................................................................0.25g
Preparation of Rifampicin Solution: Add rifampicin to 70.0mL
ethanol. Mix thoroughly. Add distilled/deionized water to bring vol-
ume to 100.0mL. Mix thoroughly. Filter sterilize.
Ferrous Sulfate, Pyruvate, Metabisulfite Solution:
Composition per 100.0mL:
FeSO 4 ..........................................................................................6.25g
Na-pyruvate ................................................................................6.25g
Na-metabisulfite..........................................................................6.25g
Preparation of Ferrous Sulfate, Pyruvate, Metabisulfite So-
lution: Add Na-pyruvate to 20mL distilled/deionized water. Mix thor-
oughly. Add Na-metabisulfite and FeSO 4 . Bring volume to 100.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except cefoperazone
solution, amphotericin B solution, rifampicin solution, ferrous sulfate
pyruvate metabisulfide solution, and horse blood, to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°C. Aseptically add 4.0mL cefoperazone solution, 4.0mL ampho-
tericin B solution, 4.0mL rifampicin solution, 4.0mL ferrous sulfate
pyruvate metabisulfide solution, and 50.0mL lysed horse blood. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the isolation and cultivation of Campylobacter spp.