Freezing Medium

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Freezing Medium

培养基配方

Composition per 11.5mL:
Bolton broth base.......................................................................9.5mL
Fetal bovine serum.....................................................................1.0mL
Glycerol solution .......................................................................1.0mL
Bolton Broth Base:
Composition per liter:
Peptone .......................................................................................10.0g
Lactalbumin hydrolysate ..............................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................5.0g
α-Ketoglutarate.............................................................................1.0g
Na-pyruvate ..................................................................................0.5g
Na-metabisulfite ...........................................................................0.5g
Na 2 CO 3 .........................................................................................0.6g
Hemin .........................................................................................0.01g
Preparation of Bolton Broth Base: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Glycerol Solution:
Composition  per 100.0mL:
Glycerol ......................................................................................10.0g
Preparation of Glycerol Solution: Add glycerol to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Preparation of Medium: Aseptically combine 9.5mL Bolton broth base, 1.0mL sterile glycerol solution, and 1.0mL filter sterilized fetal bovine serum. Mix thoroughly.
Use: For the preservation of Campylobacter spp.