Freezing Agar

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Freezing Agar

培养基配方

Composition per liter:
Potatoes, unpeeled ....................................................................200.0g
Agar ............................................................................................20.0g
Glucose .........................................................................................8.0g
Yeast extract..................................................................................1.0g
Activated carbon...........................................................................0.5g
Preparation of Medium: Thinly slice potatoes. Add to 500.0mL of tap water. Autoclave for 15 min at 15 psi pressure–121°C. Mash the potatoes in their liquid. Filter solids through cheesecloth. Add agar, glucose, yeast extract, and activated carbon to filtrate. Mix thoroughly. Bring volume to 1.0L with distilled/deionized water. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Amauroascus species, Aphanoascus cinnabarinus, Arachniotus flavoluteus, Arachniotus hebridensis, Arachnotheca albicans, Auxarthron thaxteri, Auxarthron zuffianum, Dichotomomyces cejpii, Disarticulatus devroeyi, Disarticulatus indicus, Eleutherascus lectardii, Epidermophyton floccosum, Geomyces pannorus, Gymnascella citrina, Gymnoascoideus petalosporus, Gymnoascus intermedius, Kuehniella racovitzae, Lasiobolidium orbiculoides, Macronodus bifurcatus, Microascus trigonosporus, Onygena corvina, Onygena equina, Pectinotrichum llanense, Plunkettomyces littoralis, Preussia typharum, Pseudeurotuim desertorum, Pseudeurotuim ovalis, Pseudoarachniotus roseus, Pseudoarachniotus ruber, Pseudoarachniotus trochleosporus, Rollandina capitata, Rollandina hyalinospora, and Shanorella spirotricha.