Frankia Agar

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

培养基配方

Composition per liter:
Starch, soluble.............................................................................20.0g
Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
N-Z-Amine™................................................................................5.0g
Yeast extract..................................................................................5.0g
CaCO 3 ...........................................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 7.0 with NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Frankia alni.