Francisella tularensis Isolation Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Francisella tularensis Isolation Medium 

培养基配方 

Composition per liter:
Agar ............................................................................................10.0g
Glucose .......................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Peptic digest of animal tissue......................................................10.0g
L -Cysteine·HCl·H 2 O .....................................................................5.0g
NaCl..............................................................................................5.0g
Sodium thioglycolate....................................................................2.0g
Glucose .........................................................................................1.0g
Thiamine·HCl ............................................................................5.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except agar, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Add agar. Gently heat and bring to boiling. Autoclave for 20 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri dishes.
Use: For the isolation and cultivation of Francisella tularensis.