Folic Acid HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Folic Acid HiVeg Medium 

培养基配方

Composition per liter:
Sodium citrate.............................................................................52.0g
Glucose.......................................................................................40.0g
Plant acid hydrolysate, vitamin free ...........................................10.0g
K 2 HPO 4 .........................................................................................6.4g
L -Cystine hydrochloride .............................................................0.76g
L -Asparagine.................................................................................0.6g
MgSO 4 ..........................................................................................0.4g
L -Tryptophan ................................................................................0.2g
Polysorbate 80 ..............................................................................0.1g
FeSO 4 ..........................................................................................0.02g
NaCl............................................................................................0.02g
Xanthine......................................................................................0.02g
MnSO 4 ........................................................................................0.02g
Adenine sulfate...........................................................................0.01g
Guanine hydrochloride ...............................................................0.01g
Uracil..........................................................................................0.01g
Glutathione ................................................................................5.2mg
Pyridoxine hydrochloride..........................................................4.0mg
p-Aminobenzoic acid (PABA)...................................................1.0mg
Riboflavin .................................................................................1.0mg
Nicotinic acid.............................................................................0.8mg
Calcium pantothenate ................................................................0.8mg
Thiamine hydrochloride.............................................................0.4mg
Biotin.......................................................................................0.02mg
pH 6.8 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent mixing. Cool to 45°–50°C. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For the microbiological assaying of folic acid using Enterococcus hirae as the test organism.