Folic Acid Culture HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Folic Acid Culture HiVeg Agar 

培养基配方

Composition per liter:
Plant hydrolysate No. 4...............................................................15.0g
Agar ............................................................................................10.0g
Glucose .......................................................................................10.0g
Tomato juice..................................................................................5.0g
Yeast extract..................................................................................5.0g
KH 2 PO 4 .........................................................................................2.0g
Polysorbate 80...............................................................................1.0g
pH 6.7 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent mixing. Cool to 45°–50°C. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 5 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the microbiological assaying of folic acid using Enterococcus hirae as the test organism.