Folic Acid Casei Medium with Chloramphenicol

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Folic Acid Casei Medium with Chloramphenicol 

培养基配方

Composition per liter:
Glucose.......................................................................................40.0g
Sodium acetate............................................................................40.0g
Pancreatic digest of casein, charcoal treated ..............................10.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
L- Asparagine.................................................................................0.6g
L- Cysteine·HCl·H 2 O......................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Sorbitan monooleate complex ......................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.02g
NaCl............................................................................................0.02g
Xanthine......................................................................................0.02g
MnSO 4 ·7H 2 O............................................................................0.015g
Adenine sulfate...........................................................................0.01g
Guanine·HCl...............................................................................0.01g
Uracil ..........................................................................................0.01g
Glutathione, reduced..................................................................5.0mg
Pyridoxine·HCl..........................................................................4.0mg
p-Aminobenzoic acid.................................................................2.0mg
Riboflavin..................................................................................1.0mg
Calcium pantothenate ................................................................0.8mg

Niacin.........................................................................................0.8mg
Thiamine·HCl ............................................................................0.4mg
Biotin .......................................................................................0.02mg
Chloramphenicol solution........................................................10.0mL
Folic acid solution....................................................................10.0mL
pH 6.7 ± 0.1 at 25°C
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol.........................................................................0.02g
Preparation of Chloramphenicol Solution: Add chloramphenicol to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Folic Acid Solution:
Composition per 10.0mL:
Folic acid.....................................................................................0.1μg
Preparation of Folic Acid Solution: Add folic acid to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except chloramphenicol solution and folic acid solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Aseptically add 10.0mL of sterile chloramphenicol solution and 10.0mL of sterile folic acid solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Lactobacillus casei.