Folic Acid Casei Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

Folic Acid Casei Medium 

培养基配方

Composition per liter:
Glucose .......................................................................................40.0g
Sodium acetate............................................................................40.0g

Pancreatic digest of casein, charcoal treated ..............................10.0g
K 2 HPO 4 .........................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
L- Asparagine.................................................................................0.6g
L- Cysteine·HCl·H 2 O......................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.4g
L- Tryptophan.................................................................................0.2g
Sorbitan monooleate complex ......................................................0.1g
FeSO 4 ·7H 2 O................................................................................0.02g
NaCl............................................................................................0.02g
Xanthine......................................................................................0.02g
MnSO 4 ......................................................................................0.015g
Adenine sulfate...........................................................................0.01g
Guanine·HCl...............................................................................0.01g
Uracil ..........................................................................................0.01g
Glutathione, reduced..................................................................5.0mg
Pyridoxine·HCl..........................................................................4.0mg
p-Aminobenzoic acid.................................................................2.0mg
Riboflavin..................................................................................1.0mg
Calcium pantothenate ................................................................0.8mg
Niacin.........................................................................................0.8mg
Thiamine·HCl ............................................................................0.4mg
Biotin.......................................................................................0.02mg
pH 6.7 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling with frequent mixing. Cool to 45°–50°C. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 10 min at 15 psi pressure–121°C.
Use: For assaying concentrations of folic acid, especially folic acid in blood serum, using Lactobacillus casei subspecies rhamnosus as the test organism. The extent of growth of Lactobacillus casei is proportional to the amount of folic acid in the test substance.