Fluid Thioglycolate Medium

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Fluid Thioglycolate Medium without Glucose or E h Indicator

培养基配方

Composition per liter:
Pancreatic digest of casein..........................................................20.0g
NaCl..............................................................................................2.5g
Agar ............................................................................................0.75g
L- Cystine .......................................................................................0.5g
Sodium thioglycolate....................................................................0.5g
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems .
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically distribute into sterile screw-capped tubes or flasks.
Use:  For cultivation of anaerobic bacteria. For use as a basal medium in carbohydrate fermentation tests for differentiating anaerobic bacteria. For carbohydrate fermentation tests, 1.0 mL of a 10% filter-sterilized carbohydrate solution is aseptically added to 9.0mL of fluid thioglycolate medium.