Fluid Thioglycolate Medium with Rabbit Serum

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Fluid Thioglycolate Medium with Rabbit Serum

培养基配方

Composition per liter:
Pancreatic digest of casein..........................................................15.0g
Glucose .........................................................................................5.5g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Agar ............................................................................................0.75g
L- Cystine .......................................................................................0.5g
Sodium thioglycolate....................................................................0.5g
Resazurin ...................................................................................1.0mg
Rabbit serum..........................................................................100.0mL
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components, except rabbit serum, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 9.0mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. If medium becomes oxidized before use (resazurin turns red), heat in a boiling water bath to expel absorbed O 2 . Cool to 25°C. Immediately prior to inoculation, aseptically and anaerobically add 1.0mL of sterile rabbit serum to each tube. Mix thoroughly.
Use:  For the cultivation of anaerobic, microaerophilic, and aerobic microorganisms. For use in the sterility testing of a variety of specimens.