EE Broth (Enterobacteriaceae Enrichment Broth)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EE Broth
(Enterobacteriaceae Enrichment Broth)

培养基配方
Composition per liter:
Ox bile ....................................................................20.0g
Peptone ....................................................................10.0g
Na2HPO4 ....................................................................6.45g
Glucose......................................................................5.0g
KH2PO4 ......................................................................2.0g
Brilliant Green...........................................................0.0135g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks
in 100.0mL volumes. Gently heat at 100°C for 30 min. Do not auto-
clave. Cool rapidly to 25°C.
Use: For the cultivation and enrichment of members of the Enterobac-
teriaceae in the examination of foods and animal feed. Used in con-
junction with tryptone soy broth. Bacteria belonging to the Enterobac-
teriaceae turn this medium turbid and yellow-green.