Edwards Medium, Modified

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Edwards Medium, Modified

培养基配方
Composition per 1060.0mL:
Agar........................................................................15.0g
Lab Lemco powder............................................................10.0g
Peptone ....................................................................10.0g
NaCl.........................................................................5.0g
Esculin .....................................................................1.0g
Tl2SO4 .....................................................................0.33g
Crystal Violet............................................................0.0013g
Blood, bovine or sheep.....................................................60.0mL
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Caution: Thallous sulfate is toxic.
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen-
tly heat while stirring and bring to boiling. Autoclave for 20 min at 15
psi pressure–121°C. Cool to 50°C. Aeptically add 60.0mL sterile bo-
vine or sheep blood. Mix thoroughly. Pour into sterile Petri dishes.
Use:  For the rapid isolation of Streptococcus agalactiae and other
streptococci involved in bovine mastitis. Esculin differentiates the neg-
ative Streptococcus agalactiae, which form blue colonies, from escu-
lin-positive Group D streptococci, which form black colonies.