EC Broth (Escherichia coli Broth) (EC Medium) (BAM M49)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EC Broth
(Escherichia coli Broth)
(EC Medium)
(BAM M49)

培养基配方
Composition per liter:
Pancreatic digest of casein................................................20.0g
Lactose.....................................................................5.0g
NaCl........................................................................5.0g
K2HPO4 .....................................................................4.0g
KH2PO4 .....................................................................1.5g
Bile salts No.3............................................................1.12g
Novobiocin solution.......................................................10.0mL
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid.
Novobiocin Solution:
Composition per 50.0mL:
Novobiocin ................................................................0.1g
Preparation of Novobiocin Solution: Add novobiocin to dis-
tilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except novobiocin so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 10.0mL novobiocin solution. Mix thoroughly.
Aseptically distribute to sterile tubes or flasks.
Use: A selective enrichment broth for the growth of E. coli O157 from
food and environmental samples.