EC Broth (Escherichia coli Broth) (EC Medium)

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EC Broth
(Escherichia coli Broth)
(EC Medium)

培养基
Composition per liter:
Pancreatic digest of casein.................................................20.0g
Lactose......................................................................5.0g
NaCl.........................................................................5.0g
K2HPO4 ......................................................................4.0g
Bile salts mixture...........................................................1.5g
KH2PO4 ......................................................................1.5g
pH 6.9 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube. Autoclave for 12 min at 15
psi pressure–121°C. Cool broth as quickly as possible.
Use: For the cultivation and differentiation of coliform bacteria at 37°C
and of Escherichia coli at 45.5°C.