Dextrose Proteose Peptone HiVeg Agar Base with Tellurite and Blood

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose Proteose Peptone HiVeg Agar Base
with Tellurite and Blood

培养基配方
Composition per liter:
Plant peptone No.3......................................................20.0g
Agar ...................................................................15.0g
NaCl.....................................................................5.0g
Glucose .................................................................2.0g
Sheep blood, defibrinated..............................................50.0mL
Tellurite solution......................................................2.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium, without tellurite or blood, is available as a pre-
mixed powder from HiMedia.
Tellurite Solution:
Composition per 10.0mL:
K2TeO3 ..............................................................0.1g
Preparation of Tellurite Solution: Add K2TeO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium:  Add components, except tellurite solu-
tion and blood, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add
2.0mL of sterile tellurite soltuion and 50.0mL of sterile defibrinated
blood. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of a wide variety of microor-
ganisms. For use as a base for the preparation of blood agar and for
general laboratory procedures. For the isolation of Corynebacterium
diphtheriae.