Dextrose Proteose No. 3 Agar

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose Proteose No.3 Agar

培养基配方
Composition per liter:
Proteose peptone No.3.........................................................20.0g
Agar .........................................................................13.0g
NaCl...........................................................................5.0g
Glucose .......................................................................2.0g
Tellurite blood solution.....................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Tellurite Blood Solution:
Composition per 60.0mL:
Sheep blood, defibrinated......................................................50.0mL
Chapman tellurite solution.....................................................10.0mL
Preparation of Tellurite Blood Solution: Aseptically combine
10.0mL of Chapman tellurite solution with 50.0mL of sterile, defibri-
nated sheep blood. Mix thoroughly.
Chapman Tellurite Solution:
Composition per 100.0mL:
K2TeO3 .........................................................................1.0g
Preparation of Chapman Tellurite Solution: Add K2TeO3 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except tellurite blood
solution, to distilled/deionized water and bring volume to 940.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 75°–80°C. Aseptically add 50.0mL
of sterile tellurite blood solution. Mix thoroughly. Maintain at 75°–
80°C for 10–15 min or until the agar becomes chocolatized. Cool slow-
ly to 50°C. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For propagating pure cultures of Neisseria gonorrhoeae and
other fastidious microorganisms.