Desulfotomaculum halophilum Medium (DSMZ Medium 815)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfotomaculum halophilum Medium
(DSMZ Medium 815)

培养基配方
Composition per liter:
NaCl.......................................................................40.0g
MgCl2 ·6H2O................................................................8.0g
CaCl2 ·2H2O................................................................6.0g
Na-pyruvate ................................................................3.6g
Na2SO4 .....................................................................3.0g
MOPS........................................................................3.0g
KCl.........................................................................2.0g
Yeast extract...............................................................1.0g
NH4Cl ......................................................................0.3g
KH2PO4 .....................................................................0.2g
SrCl2 ·6H2O................................................................0.1g
Resazurin..................................................................0.5mg
NaHCO3  solution..........................................................50.0mL
Trace elements solution SL-12..............................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-12:
Composition per liter:
Na2 -EDTA...................................................................5.2g
FeCl2 ·4H2O................................................................1.5g
CoCl2 ·6H2O.............................................................190.0mg
MnCl2 ·4H2O ............................................................100.0mg
ZnCl2 ....................................................................70.0mg
Na2MoO4 ·2H2O............................................................36.0mg
NiCl2 ·6H2O..............................................................24.0mg
H3BO3 .....................................................................6.0mg
CuCl2 ·2H2O...............................................................2.0mg
HCl (25% solution)........................................................10.0mL
Preparation of Trace Elements Solution SL-12: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Adjust pH to 6.0. Sparge with 80% N2 + 20% CO2 . Autoclave
for 15 min at 15 psi pressure–121°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ...................................................................10.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 80% N2 + 20% CO2 . Filter sterilize.
Preparation of Medium: Prepare and dispense medium under
100% N2 . Add components, except NaHCO3 solution, to distilled/de-
ionized water and bring volume to 950.0mL. Mix thoroughly. Sparge
with 100% N2 . Adjust pH to 6.0. Dispense under 100% N2  into tubes
or bottles. Autoclave for 30 min at 105°C. Cool to 25°C. Aseptically
and anaerobically add sterile NaHCO3  solution, 0.5mL per 10.0mL
medium. Final pH is 7.2.
Use:  For the cultivation of Desulfocella halophila DSM 11763.