Desulfotomaculum halophilum Medium (DSMZ Medium 815)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfotomaculum halophilum Medium
(DSMZ Medium 815)

培养基配方
Composition per liter:
Iron, powder...............................................................150.0g
NaCl........................................................................40.0g
MgCl2 ·6H2O ................................................................8.0g
CaCl2 ·2H2O ................................................................6.0g
Na-lactate...................................................................3.6g
Na2SO4 ......................................................................3.0g
MOPS.........................................................................3.0g
KCl..........................................................................2.0g
Yeast extract................................................................1.0g
NH4Cl .......................................................................0.3g
KH2PO4 ......................................................................0.2g
SrCl2 ·6H2O.................................................................0.1g
Resazurin ..................................................................0.5mg
NaHCO3  solution ..........................................................50.0mL
Trace elements solution SL-12 ..............................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-12:
Composition per liter:
Na2 -EDTA...................................................................5.2g
FeCl2 ·4H2O................................................................1.5g
CoCl2 ·6H2O ............................................................190.0mg
MnCl2 ·4H2O.............................................................100.0mg
ZnCl2 ....................................................................70.0mg
Na2MoO4 ·2H2O ...........................................................36.0mg
NiCl2 ·6H2O..............................................................24.0mg
H3BO3 .....................................................................6.0mg
CuCl2 ·2H2O ..............................................................2.0mg
HCl (25% solution)........................................................10.0mL
Preparation of Trace Elements Solution SL-12: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Adjust pH to 6.0. Sparge with 80% N2 + 20% CO2 . Autoclave
for 15 min at 15 psi pressure–121°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ...................................................................10.0g
Preparation of NaHCO3 Solution:  Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Sparge
with 80% N2 + 20% CO2 . Filter sterilize.
Preparation of Medium: Prepare and dispense medium under
100% N2 . Add components, except iron and NaHCO3 solution, to dis-
tilled/deionized water and bring volume to 950.0L. Mix thoroughly.
Sparge with 100% N2 . Adjust pH to 6.0. Dispense under 100% N2  into
tubes or bottles containing 1.5g iron per 10.0mL medium. Autoclave
for 30 min at 105°C. Cool to 25°C. Aseptically and anaerobically add
sterile NaHCO3  solution, 0.5mL per 10.0mL medium. Final pH is 7.2.
Use:  For the cultivation of Desulfotomaculum halophilum and Desulfo-
cella halophila.