Desulfococcus amylolyticus Medium

[所属分类:培养基配方] [发布时间:2020-6-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfococcus amylolyticus Medium

培养基配方
Composition per 1011.0mL:
Sulfur, powdered.............................................................10.0g
Starch........................................................................5.0g
NaHCO3 .......................................................................0.8g
MgCl2 ·6H2O .................................................................0.7g
Na2S·9H2O....................................................................0.5g
NaCl..........................................................................0.5g
CaCl2 ·2H2O ................................................................0.44g
KCl..........................................................................0.33g
KH2PO4 ......................................................................0.33g
NH4Cl .......................................................................0.33g
Yeast extract.................................................................0.2g
Resazurin ...................................................................1.0mg
Vitamin solution............................................................10.0mL
Trace elements solution SL-10 ...............................................1.0mL
pH 6.2–6.4 at 25°C
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl...............................................................10.0mg
Calcium  DL -pantothenate.....................................................5.0mg
Lipoic acid ..................................................................5.0mg
Nicotinic acid................................................................5.0mg
p-Aminobenzoic acid...........................................................5.0mg
Riboflavin ...................................................................5.0mg
Thiamine-HCl..................................................................5.0mg
Biotin .......................................................................2.0mg
Folic acid....................................................................2.0mg
Vitamin B 12 .................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Gas under
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O...................................................................1.5g
CoCl2 ·6H2O ...............................................................190.0mg
MnCl2 ·4H2O................................................................100.0mg
ZnCl2 .......................................................................70.0mg
Na2MoO4 ·2H2O ..............................................................36.0mg
NiCl2 ·6H2O.................................................................24.0mg
H3BO3 ........................................................................6.0mg
CuCl2 ·2H2O .................................................................2.0mg
HCl (25% solution)...........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl. Mix thoroughly. Add distilled/deionized water and
bring volume to 1.0L. Add remaining components. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gas under 80% N2 +
20% CO2 . Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Desulfurococcus amylolyt-
icus.