Desulfocapsa sulfoexigens Medium (DSMZ Medium 195b)

[所属分类:培养基配方] [发布时间:2020-6-5] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfocapsa sulfoexigens Medium
(DSMZ Medium 195b)

培养基配方
Composition per 1001.0mL:
Solution A................................................................890.0mL
Solution C................................................................100.0mL
Solution D.................................................................10.0mL
Solution B (Trace elements solution SL-10)..................................1.0mL
pH 7.2 ± 0.2 at 25°C
Solution A:
NaCl........................................................................21.0g
MgCl2 ·6H2O.................................................................3.1g
Na2SO4 ......................................................................3.0g
FeCl3 ·6H2O.................................................................2.7g
KCl..........................................................................0.5g
NH4Cl .......................................................................0.3g
KH2PO4 ......................................................................0.2g
CaCl2 ·2H2O ...............................................................0.15g
Resazurin ..................................................................1.0mg
Preparation of Solution A: Dissolve 2.7g FeCl3 ·6H2O in 890.0ml
distilled/deionized water. Adjust pH to 7 with 1N NaOH. Add remain-
ing components. Mix thoroughly. Sparge with 80% N2  + 20% CO2 gas
mixture.
Solution B (Trace Elements Solution SL-10):
Composition per liter:
FeCl2 ·4H2O.................................................................1.5g
CoCl2 ·6H2O..............................................................190.0mg
MnCl2 ·4H2O .............................................................100.0mg
ZnCl2 .....................................................................70.0mg
Na2MoO4 ·2H2O.............................................................36.0mg
NiCl2 ·6H2O...............................................................24.0mg
H3BO3 ......................................................................6.0mg
CuCl2 ·2H2O................................................................2.0mg
HCl (25% solution).........................................................10.0mL
Preparation of Solution B (Trace Elements Solution SL-10):
Add FeCl2 ·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis-
tilled/deionized water and bring volume to 1.0L. Add remaining com-
ponents. Mix thoroughly. Sparge with 100% N2 . Autoclave for 15 min
at 15 psi pressure–121°C.
Solution C:
Composition per 100.0mL:
NaHCO3 .....................................................................5.0g
Preparation of Solution C: Add NaHCO3 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Flush with 80% N2  + 20% CO2 to remove dissolved oxygen.
Solution D:
Composition per 10.0mL:
Na-thiosulfate ...............................................................5.0g
Preparation of Solution D: Add Na-thiosulfate to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril-
ize. Flush with 80% N2  + 20% CO2 to remove dissolved oxygen.
Preparation of Medium: Gently heat solution A and bring to boil-
ing. Boil solution A for a few minutes. Cool to room temperature. Gas
with 80% N2  + 20% CO2 gas mixture to reach a pH below 6. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to room temperature. Se-
quentially add 1.0mL solution B, 100.0mL solution C, and 10.0mL so-
lution D. Adjust pH to 7.2 with sodium bicarbonate or sodium
carbonate. Distribute anaerobically under 80% N2  + 20% CO2 into ap-
propriate vessels. Alternately distribute solution A to tubes prior to au-
toclaving. Dispense 8.9 mL amounts under 80% N2  + 20% CO2 into
Hungate tubes. Seal and autoclave for 15 min at 15 psi pressure–
121°C. Before use aseptically add appropriate amounts of remaining
solutions to each tube from sterile anaerobic solutions.
Use: For the cultivation of Desulfocapsa sulfoexigens.