Deoxycholate Lactose Sucrose Sorbitol Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Lactose Sucrose Sorbitol Agar

培养基配方
Composition per liter:
Sodium citrate.................................................................20.0g
Agar ..........................................................................15.0g
D- Sorbitol ...................................................................10.0g
Lactose........................................................................10.0g
Sucrose.........................................................................5.0g
Pancreatic digest of casein.....................................................5.0g
Yeast extract...................................................................5.0g
Sodium deoxycholate.............................................................2.5g
Ferric citrate..................................................................1.0g
Neutral Red....................................................................0.02g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not overheat. Adjust pH to 7.4. Do not autoclave. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Hafnia species.