Deoxycholate Lactose HiVeg Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Lactose HiVeg Agar

培养基配方
Composition per liter:
Agar ............................................................................15.0g
Plant special peptone ...........................................................10.0g
Lactose..........................................................................10.0g
NaCl..............................................................................5.0g
Sodium citrate....................................................................2.0g
Synthetic detergent No. III.......................................................0.5g
Neutral Red......................................................................0.03g
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes. Dry the agar surface before use.
Use: For the selective isolation, cultivation, and differentiation of enteric
pathogens, especially Salmonella and Shigella species. Lactose-ferment-
ing bacteria appear as pink colonies that may or may not be surrounded by
a zone of precipitated deoxycholate. Nonlactose-fermenting bacteria
appear as colorless colonies that are surrounded by a clear orange-yellow
zone. Also used for the enumeration of coliform bacteria from water, milk,
and dairy products.