Deoxycholate Citrate Agar, Hynes

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Citrate Agar, Hynes
Composition per liter:
Agar.........................................................................12.0g
Lactose......................................................................10.0g
Sodium citrate................................................................8.5g
Na2S2O3 ·5H2O................................................................5.4g
Beef extract powder...........................................................5.0g
Peptone ......................................................................5.0g
Sodium deoxycholate...........................................................5.0g
Ferric citrate................................................................1.0g
Neutral Red..................................................................0.02g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes. Dry the agar surface before use.
Use: For the selective isolation, cultivation, and differentiation of
enteric pathogens, especially Salmonella and Shigella species. Lac-
tose-fermenting bacteria appear as pink colonies that may or may not
be surrounded by a zone of precipitated deoxycholate. Nonlactose-fer-
menting bacteria appear as colorless colonies that are surrounded by a
clear orange-yellow zone.