Deoxycholate Citrate Agar, HiVeg

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Citrate Agar, HiVeg

培养基配方
Composition per liter:
Sodium citrate...............................................................20.0g
Agar ........................................................................13.5g
Plant peptone No.3...........................................................13.0g
Plant infusion...............................................................10.0g
Lactose......................................................................10.0g
Synthetic detergent No. III...................................................2.0g
Ferric ammonium citrate.......................................................2.0g
Neutral Red..................................................................0.02g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes. Dry the agar surface before use. Avoid excessive heating as it
is detrimental to the medium.
Use: For the selective isolation and cultivation of enteric pathogens,
especially Salmonella and Shigella species.