Deoxycholate Citrate Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Citrate Agar

培养基配方
Composition per liter:
Sodium citrate.................................................................20.0g
Agar...........................................................................17.0g
Lactose........................................................................10.0g
Meat, solids from infusion.....................................................10.0g
Peptic digest of animal tissue ................................................10.0g
Sodium deoxycholate.............................................................5.0g
Ferric citrate..................................................................1.0g
Neutral Red....................................................................0.02g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes. Dry the agar surface before use.
Use: For the selective isolation and cultivation of enteric pathogens,
especially Salmonella and Shigella species.