Deoxycholate Citrate Agar

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Deoxycholate Citrate Agar

培养基配方
Composition per liter:
Sodium citrate...................................................................50.0g
Agar.............................................................................15.0g
Lactose..........................................................................10.0g
Beef extract......................................................................5.0g
Peptone ..........................................................................5.0g
Na2S2O3 ·5H2O....................................................................5.0g
Sodium deoxycholate...............................................................2.5g
Ferric citrate....................................................................1.0g
Neutral Red.....................................................................0.025g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri
dishes. Dry the agar surface before use.
Use: For the selective isolation and cultivation of enteric pathogens,
especially Salmonella and Shigella species.