​Davis Supplemented Minimal Medium

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis Supplemented Minimal Medium

培养基配方
Composition per liter:
Agar.........................................................................15.0g
K2HPO4 .......................................................................7.0g
KH2PO4 .......................................................................3.0g
Casein hydrolysate............................................................2.0g
Yeast extract.................................................................2.0g
(NH4)2SO4 ....................................................................1.0g
Sodium citrate·3H2O..........................................................0.5g
MgSO4 ·7H2O..................................................................0.1g
Glucose solution ...........................................................20.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution
Composition per 100.0mL:
Glucose......................................................................10.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 20.0mL of sterile glucose
solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation and maintenance of Escherichia coli.