Davis and Mingioli Medium with Vitamin B 1 and Asparagine

[所属分类:培养基配方] [发布时间:2020-6-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Davis and Mingioli Medium

培养基配方
with Vitamin B 1 and Asparagine
Composition per liter:
K2HPO4 ......................................................................7.0g
KH2PO4 ......................................................................3.0g
(NH4)2SO4 ...................................................................1.0g
Sodium citrate·3H2O.........................................................0.5g
L- Asparagine................................................................0.4g
MgSO4 ·7H2O.................................................................0.1g
Vitamin B 1 ................................................................0.1mg
Glucose solution............................................................1.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose......................................................................20.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 999.0mL. Gently
heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 25°C. Aseptically add 1.0mL of sterile glucose solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Escherichia coli.