CYU 2%

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CYU 2%

培养基配方
Composition per liter:
Basal solution...........................................................960.0mL
Yeast extract solution....................................................30.0mL
Trace metal solution......................................................10.0mL
Basal Solution:
Composition per 960.0mL:
Glucose....................................................................20.0g
K2HPO4 .....................................................................9.2g
Urea........................................................................5.0g
(NH4)2S04 ..................................................................2.2g
KH2PO4 .....................................................................1.3g
Trisodium citrate·2H2O.....................................................1.0g
MgSO4 ·7H2O solution.....................................................1.66mL
MgSO4 ·7H2O Solution:
Composition per 10.0mL:
MgSO4 ·7H2O...............................................................2.5g
Preparation of MgSO4 ·7H2O Solution: Add MgSO4 ·7H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly.
Preparation of Basal Solution: Add components to distilled/de-
ionized water and bring volume to 960.0mL. Mix thoroughly. Filter
sterilize.
Yeast Extract Solution:
Composition per 100.0mL:
Yeast extract...............................................................10.0g
Preparation of Yeast Extract Solution : Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
© 2010 by Taylor and Francis Group, LLC
Czapek Agar with 20% Sucrose  479
Trace Metal Solution:
Composition per liter:
CaCl2 ·2H2O...............................................................3972.0mg
FeSO4 ·7H2O...............................................................1250.0mg
H3BO3 .....................................................................1140.0mg
ZnSO4 ·7H2O................................................................882.0mg
CuCl2 ·5H2O ...............................................................157.0mg
MnCl2 ·4H2O................................................................140.0mg
NaMoO4 ·2H2O...............................................................119.0mg
Vanadyl sulfate·2H2O ......................................................100.0mg
CsCl2 ·6H2O.................................................................49.0mg
FeCl3 ·6H2O.................................................................29.0mg
CdSO4 ·8H2O.................................................................10.0mg
Ni(NO3)2 ·6H2O..............................................................10.0mg
HCl, concentrated ...........................................................10.0mL
Preparation of Trace Metal Solution : Add 10.0mL of concen-
trated HCl to 900.0mL of distilled/deionized water. Mix thoroughly.
Add remaining components in the order shown. Mix thoroughly after
adding each component. Bring volume to 990.0mL with distilled/de-
ionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Aseptically combine 960.0mL of sterile
basal solution, 30.0mL of sterile yeast extract solution, and 30.0mL of
sterile trace metal solution. Aseptically distribute into sterile tubes or
flasks.
Use: For the cultivation of Neospongicoccum excentricum.