Cystine Tellurite Blood Agar

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cystine Tellurite Blood Agar

培养基配方
Composition per 120.0mL:
Heart infusion agar.......................................................100.0mL
K2TeO3 solution............................................................15.0mL
Sheep blood.................................................................5.0mL
L- Cystine .................................................................5.0mg
pH 7.4 ± 0.2 at 25°C
Heart Infusion Agar:
Composition per liter:
Beef heart, infusion from...................................................500.0g
Agar.........................................................................20.0g
Tryptose.....................................................................10.0g
Yeast extract.................................................................5.0g
NaCl..........................................................................5.0g
Preparation of Heart Infusion Agar:  Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
K2TeO3 Solution:
Composition per 100.0mL:
K2TeO3 .........................................................................0.3g
Preparation of K2TeO3 Solution: Add K2TeO3 to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add sterile K2TeO3 solution, sterile, de-
fibrinated sheep blood, and sterile, solid  L- cystine to sterile, cooled
heart infusion agar. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the isolation, differentiation, and cultivation of Corynebacte-
rium diphtheriae. Corynebacterium diphtheriae appears as dark gray to
black colonies.