Cytophaga Medium

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga Medium

培养基配方
Composition per liter:
NaCl..................................................................30.0g
Agar..................................................................15.0g
KH2PO4 ................................................................1.0g
NH4Cl .................................................................1.0g
Yeast extract..........................................................1.0g
MgSO4 .................................................................0.5g
CaCl2 ................................................................0.04g
FeCl3 ·6H2O.........................................................1.25mg
NaHCO3 solution.....................................................100.0mL
Glucose solution.....................................................10.0mL
Na2S·9H2O solution...................................................1.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose...............................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ..................................................................5.0g
Preparation of NaHCO3 Solution: Add NaHCO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Na2S·9H2O Solution:
Composition per 100.0mL:
Na2S·9H2O................................................................10.0g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except NaHCO3 solu-
tion, glucose solution, and Na2S·9H2O solution, to distilled/deionized
water and bring volume to 889.0mL. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C. Aseptically add sterile NaHCO3 solution,
sterile glucose solution, and sterile Na2S·9H2O solution. Mix thorough-
ly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Cytophaga agarovorans.