Cytophaga Marine Medium (DSMZ Medium 172)

[所属分类:培养基配方] [发布时间:2020-5-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cytophaga Marine Medium
(DSMZ Medium 172)

培养基配方
Composition per liter:
NaCl.......................................................................24.7g
Agar ......................................................................15.0g
MgSO4 ·7H2O................................................................6.3g
MgCl2 ·6H2O................................................................4.6g
CaCl2 ·2H2O................................................................1.2g
Yeast extract...............................................................1.0g
Tryptone....................................................................1.0g
KCl.........................................................................0.7g
Sodium bicarbonate solution...............................................10.0mL
Calcium chloride solution.................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Sodium Bicarbonate Solution:
Composition per 10.0mL:
NaHCO3 .....................................................................0.2g
Preparation of Sodium Bicarbonate Solution: Add NaHCO 3 to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Calcium Chloride Solution:
Composition per 10.0mL:
CaCl2 ·2H2O................................................................1.2g
Preparation of Calcium Chloride Solution: Add CaCl2 ·2H2O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except sodium bicar-
bonate and calcium chloride solution, to distilled/deionized water and
bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–
50°C. Aseptically add 10.0mL of sterile bicarbonate solution and
10.0mL sterile calcium chloride solution. Adjust pH to 7.2. Mix thor-
oughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Cellulophaga lytica, Cy-
tophaga latercula, Marinilabilia salmonicolor, Saprospira grandis,
Cytophaga marinoflava, Persicobacter diffluens, Flammeovirga apri-
ca, Flexibacter tractuosus, Microscilla spp., Marinilabilia salmonicol-
or, Flexibacter litoralis, Flexithrix dorotheae, and Cellulophaga lytica.