CTA Medium with Yeast Extract and Rabbit Serum (Cystine Trypticase™ Agar Medium with Yeast Ex- tract and Rabbit Serum)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CTA Medium with Yeast Extract and Rabbit Serum
(Cystine Trypticase™ Agar Medium with Yeast Ex-
tract and Rabbit Serum)

培养基配方
Composition per liter:
Yeast extract..................................................................50.0g
Pancreatic digest of casein....................................................20.0g
NaCl............................................................................5.0g
Carbohydrate....................................................................5.0g
Agar............................................................................2.5g
L- Cystine......................................................................0.5g
Na2SO3 .........................................................................0.5g
Phenol Red....................................................................0.017g
Rabbit serum.................................................................250.0mL
pH 7.3 ± 0.2 at 25oC
Preparation of Medium: Add components, except rabbit serum, to
distilled/deionized water and bring volume to 750.0mL. Mix thorough-
ly. Adjust pH to 7.3. Gently heat until boiling. Autoclave for 15 min at
15 psi pressure–118°C. Cool to 50°C. Aseptically add sterile rabbit se-
rum. Mix thoroughly. Distribute into sterile tubes. Store at room tem-
perature. Do not refrigerate.
Use: For the cultivation and maintenance of fastidious microorgan-
isms, especially mycoplasmas and related microorganisms