CTA Medium (Cystine Trypticase™ Agar Medium) (Cystine Tryptic Agar)

[所属分类:培养基配方] [发布时间:2020-5-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CTA Medium
(Cystine Trypticase™ Agar Medium)
(Cystine Tryptic Agar)

培养基配方
Composition per liter:
Pancreatic digest of casein..................................................20.0g
NaCl..........................................................................5.0g
Carbohydrate..................................................................5.0g
Agar..........................................................................2.5g
L- Cystine....................................................................0.5g
Na2SO3 .......................................................................0.5g
Phenol Red..................................................................0.017g
pH 7.3 ± 0.2 at 25°C
Source: The medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3.
Gently heat until boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–118°C. Cool tubes in an upright position.
Store at room temperature.
Use: For the cultivation and maintenance of a variety of fastidious
microorganisms. For the detection of bacterial motility. Used, with
added specific carbohydrate, for fermentation reactions of fastidious
microorganisms, especially Neisseria species, pneumococci, strepto-
cocci, and nonspore-forming anaerobes.