Corynebacterium Medium with Blood (DSMZ Medium 240)

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Corynebacterium Medium with Blood
(DSMZ Medium 240)

培养基配方
Composition per liter:
Agar ........................................................................15.0g
Casein peptone, tryptic digest...............................................10.0g
Yeast extract.................................................................5.0g
Glucose ......................................................................5.0g
NaCl..........................................................................5.0g
Distilled water...........................................................1000.0mL
Sheep or horse blood, defibrinated...........................................50.0mL
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components, except sheep or horse
blood, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
sheep or horse blood. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of Streptococcus alactolyticus, Corynebacte-
rium spp., Desemzia incerta=Brevibacterium incertum, and Moraxella
bovis.