Corynebacterium Liquid Enrichment Medium

[所属分类:培养基配方] [发布时间:2020-5-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Corynebacterium Liquid
Enrichment Medium

培养基配方
Composition per 2000.0mL:
Fosfomycin..................................................................0.15g
Glucose 6-phosphate.........................................................0.03g
Solution A.................................................................985.0mL
Bovine serum...............................................................100.0mL
Nystatin solution...........................................................1.15mL
L- Cystine (1% solution) ....................................................1.0mL
Egg yolk emulsion...........................................................10 eggs
pH 7.4 ± 0.2 at 25°C
Solution A:
Composition per liter:
Meat extract ...............................................................9.0g
Proteose peptone No.3.......................................................9.0g
NaCl........................................................................2.7g
Glucose ....................................................................1.8g
Na2HPO4 ·12H2O.............................................................1.8g
K2TeO3 (2% solution)......................................................75.0mL
L- Cystine (1% solution) .................................................10.0mL
Caution: Potassium tellurite is toxic.
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 985.0mL. Mix thoroughly. Filter sterilize.
Egg Yolk Emulsion:
Composition :
Chicken egg yolks..................................................................9
Whole chicken egg..................................................................1
Preparation of Egg Yolk Emulsion:  Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg. Filter
sterilize.
Nystatin Solution:
Composition per 10.0mL:
Nystatin......................................................................10,000U
Preparation of Nystatin Solution: Add nystatin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
L -Cystine Solution:
Composition per 10.0mL:
L- Cystine ......................................................................0.1g
Preparation of  L -Cystine Solution: Add  L -cystine to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: To 985.0mL of sterile solution A, asepti-
cally add the remaining components. Mix thoroughly. Aseptically dis-
tribute into sterile tubes in 2.0–3.0mL volumes.
Use: For the isolation and cultivation of Corynebacterium diphthe-
riae.