Columbia Broth Base, HiVeg with SPS

[所属分类:培养基配方] [发布时间:2020-5-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Columbia Broth Base, HiVeg with SPS

培养基配方
Composition per liter:
Plant peptone No.5................................................................10.0g
Plant special peptone ............................................................10.0g
NaCl...............................................................................5.0g
Plant infusion.....................................................................3.0g
Tris (hydroxymethyl) aminomethane .................................................2.86
Glucose............................................................................2.5g
Na2CO3 ............................................................................0.6g
L -Cystine hydrochloride ..........................................................0.1g
MgSO4 .............................................................................0.1g
FeSO4 ............................................................................0.02g
SPS (sodium polystyrene sulfonate)................................................0.1mL
pH 7.5 ± 0.2 at 25°C......
Source: This medium, without SPS, is available as a premixed pow-
der from HiMedia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat until
boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Mix thoroughly.
Use: For the cultivation and isolation of fastidious bacteria from clin-
ical specimens. For blood cultures, the SPS inhibits lysozyme activity
and interferes with phagocytosis and destroys the aminoglycosides.